Tavora, R., Zhang, L., Tran, M. H., Li, H., O’Hagan, D., Pan, A., . . . Valente, S. T. (2025). Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases. Human Gene Therapy. doi:10.1089/hum.2025.011
Ma Y, Li C, Valente S. Human chromatin remodelers regulating HIV-1 transcription: a target for small molecule inhibitors. Epigenetics Chromatin. 2025 Apr 16;18(1):21. doi: 10.1186/s13072-025-00582-w. PMID: 40241225; PMCID: PMC12004603.
Gibaut QMR, Li C, Cheng A, Moranguinho I, Mori LP, Valente ST. FUBP3 enhances HIV-1 transcriptional activity and regulates immune response pathways in T cells. Mol Ther Nucleic Acids. 2025 Mar 25;36(2):102525. doi: 10.1016/j.omtn.2025.102525. PMID: 40248217; PMCID: PMC12005928.
Cleary, Megana; Ndhlovu, Lishomwa C.b; Sacha, Jonah B.a,c. Stem cell transplantation and allogeneic immunity: post treatment control or HIV cure?. Current Opinion in HIV and AIDS ():10.1097/COH.0000000000000892, October 22, 2024. | DOI: 10.1097/COH.0000000000000892
Li, S., Nguyen, K., & Yang, H. (2024). The cancer-immune setpoint in HIV cure. Cell Reports Medicine, 5(8), 100923. https://doi.org/10.1016/j.xcrm.2024.100923
Prigann, J., Tavora, R., Furler O’Brien, R.L. et al. Silencing the transcriptionally active HIV reservoir to improve treatment outcomes. Nat Microbiol (2024). https://doi.org/10.1038/s41564-024-01816-5
Jang, Y., & Kim, Y. (2024). Retro-age: A unique epigenetic biomarker of aging captured by DNA methylation states of retroelements. Aging Cell, 23(2), e14288. https://doi.org/10.1111/acel.14288
Mori, L. P., Corley, M. J., McAuley, A. T., Pang, A., Venables, T., Ndhlovu, L. C., . . . Valente, S. T. (2024). Transcriptional and methylation outcomes of didehydro-cortistatin A use in HIV-1–infected CD4<sup>+</sup> T cells. Life Science Alliance, 7(10), e202402653. doi:10.26508/lsa.202402653
Ament, S.A., Campbell, R.R., Lobo, M.K. et al. The single-cell opioid responses in the context of HIV (SCORCH) consortium. Mol Psychiatry (2024). https://doi.org/10.1038/s41380-024-02620-7
Ndukwe SO, Patel H, Shelton B, Concha-Garcia S, Dullano C, Solso S, Hendrickx S, Riggs PK, Villa TJ, Kaytes A, Taylor J, Little SJ, Lessard D, Arora AK, Costiniuk CT, Eskaf S, Smith DM, Gianella S, Dubé K. People with HIV at the end-of-life and their next-of-kin/loved ones are willing ...
Dopkins N, Singh B, Michael S, Zhang P, Marston JL, Fei T, Singh M, Feschotte C, Collins N, Bendall ML, Nixon DF. Ribosomal profiling of human endogenous retroviruses in healthy tissues. BMC Genomics. 2024 Jan 2;25(1):5. doi: 10.1186/s12864-023-09909-x. PMID: 38166631
Helena Reyes-Gopar, Jez L. Marston, Bhavya Singh, Matthew Greenig, Jonah Lin, Mario A. Ostrowski, Kipchoge N. Randall Jr., Santiago Sandoval-Motta, Nicholas Dopkins, Elsa Lawrence, Morgan M. O’Mara, Tongyi Fei, Rodrigo R. R. Duarte, Timothy R. Powell, Enrique Hernández-Lemus, Luis P. Iñiguez, Douglas F. Nixon, Matthew L. Bendall doi: https://doi.org/10.1101/2023.12.28.573568
Tuma, Jan; Chen, Yu-Ju; Collins, Michael G; Paul, Abhik; Li, Jie; Han, Hesong; Sharma, Rohit; Murthy, Niren; Lee, Hye Young Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection. Biochemistry2023 Dec 19; 62 (24) 3533-3547
Singh M, Leddy SM, Iñiguez LP, Bendall ML, Nixon DF, Feschotte C. Transposable elements may enhance antiviral resistance in HIV-1 elite controllers. bioRxiv. 2023 Dec 12:2023.12.11.571123. doi: 10.1101/2023.12.11.571123. Preprint. PMID: 38168352
3D Human Tissue Models for HIV Working Group. Electronic address: dwight.yin@nih.gov. 3D human tissue models and microphysiological systems for HIV and related comorbidities. Trends Biotechnol. 2023 Dec 8:S0167-7799(23)00299-8. doi: 10.1016/j.tibtech.2023.10.008. Epub ahead of print. PMID: 38071144.
Ndhlovu LC, Bendall ML, Dwaraka V, Pang AP, Dopkins N, Carreras N, Smith R, Nixon DF, Corley MJ. Retroelement-Age Clocks: Epigenetic Age Captured by Human Endogenous Retrovirus and LINE-1 DNA methylation states. bioRxiv [Preprint]. 2023 Dec 7:2023.12.06.570422. doi: 10.1101/2023.12.06.570422. PMID: 38106164; PMCID: PMC10723416
Boehm D, Kaehlcke K, Schnoelzer M, Ott M. Protocol for an in vitro assay to study HIV-1 Tat methylation. STAR Protoc. 2023 Nov 17;4(4):102687. doi: 10.1016/j.xpro.2023.102687. Epub ahead of print. PMID: 37979180; PMCID: PMC10694577.
Lyons, D.E.; Kumar, P.; Roan, N.R.; Defechereux, P.A.; Feschotte, C.; Lange, U.C.; Murthy, N.; Sameshima, P.; Verdin, E.; Ake, J.A.; Furler O’Brien, R.; et al. HIV-1 Remission: Accelerating the Path to Permanent HIV-1 Silencing. Viruses 2023, 15, 2171. https://doi.org/10.3390/v15112171
Preeti Moar, Thomas A. Premeaux, Andrew Atkins, Lishomwa C. Ndhlovu and Vinayaka R. Prasad
Goyal R, De Gruttola V, Gianella S, Caballero G, Porrachia M, Ignacio C, Woodworth B, Smith DM, Chaillon A. Identification of system-level features in HIV migration within a host. PLoS One. 2023 Sep 26;18(9):e0291367. doi: 10.1371/journal.pone.0291367. PMID: 37751407; PMCID: PMC10521982.
Li Z, Deeks SG, Ott M, Greene WC. Comprehensive synergy mapping links a BAF- and NSL-containing “supercomplex” to the transcriptional silencing of HIV-1. Cell Rep. 2023 Sep 26;42(9):113055. doi: 10.1016/j.celrep.2023.113055. Epub 2023 Sep 7. PMID: 37682714; PMCID: PMC10591912.
Exploiting a rodent cell block for intrinsic resistance to HIV-1 gene expression in human T cells Ryan T. Behrens rtbehrens@wisc.edu , Jyothi Krishnaswamy Rajashekar , James W. Bruce , Edward L. Evans, III , Amelia M. Hansen , Natalia Salazar-Quiroz , Lacy M. Simons , Paul Ahlquist , Judd F. Hultquist , Priti Kumar , Nathan M. Sherer nsherer@wisc.edu
Corley MJ, Pang APS, Shikuma CM, Ndhlovu LC. Cell-type specific impact of metformin on monocyte epigenetic age reversal in virally suppressed older people living with HIV. Aging Cell. 2023 Sep 7:e13926. doi: 10.1111/acel.13926. Epub ahead of print. PMID: 37675817.
Trunfio M, Chaillon A, Beliakova-Bethell N, Deiss R, Letendre SL, Riggs PK, Higgins N, Gianella S. Beyond the Syndemic of Opioid Use Disorders and HIV: The Impact of Opioids on Viral Reservoirs. Viruses. 2023 Aug 9;15(8):1712. doi: 10.3390/v15081712. PMID: 37632053; PMCID: PMC10458944.
Dubé K, Barr E, Philbin M, Perez-Brumer A, Minalga B, Peterson B, Averitt D, Picou B, Martel K, Chung C, Mejía M, Cameron M, Graham G, Dee L, Dixon Diallo D, Gordon E, Korolkova A, Dyer T, Auerbach JD, Scully E, Dong KL, Gianella S. Increasing the meaningful involvement of ...
Bianchi L, Fiorentini S, Gianella S, Gianotti S, Iadanza C, Asadi S, Saccomandi P. Measurement of Thermal Conductivity and Thermal Diffusivity of Porcine and Bovine Kidney Tissues at Supraphysiological Temperatures up to 93 °C. Sensors (Basel). 2023 Aug 2;23(15):6865. doi: 10.3390/s23156865. PMID: 37571648; PMCID: PMC10422510
Popovitz, Juliana; Sharma, Rohit; Hoshyar, Reyhane; Soo Kim, Beob; Murthy, Niren; Lee, Kunwoo Gene editing therapeutics based on mRNA delivery. Advanced drug delivery reviews2023 Sep; 200 115026
Chen, X., Wang, Y., Cappuccio, A. et al. Mapping disease regulatory circuits at cell-type resolution from single-cell multiomics data. Nat Comput Sci 3, 644–657 (2023). https://doi.org/10.1038/s43588-023-00476-5
Gibaut QMR, Mori LP, Valente ST. HIV-1 transcriptional modulation: novel host factors and prospective therapeutic strategies. Curr Opin HIV AIDS. 2023 Sep 1;18(5):264-272. doi: 10.1097/COH.0000000000000808. Epub 2023 Jul 17. PMID: 37535041; PMCID: PMC10403276.
Gaitan NC, D’Antoni ML, Acosta RK, Gianella S, Little SJ, Chaillon A. Brief Report: Comparative Analysis of Pre-existing HIV Drug Resistance Mutations in Proviral DNA Using Next-Generation Sequencing and Routine HIV RNA Genotyping. J Acquir Immune Defic Syndr. 2023 Jul 1;93(3):213-218. doi: 10.1097/QAI.0000000000003195. PMID: 36961945; PMCID: PMC10272101.
Hastie E, Moser C, Sun X, Lennox J, Hsue PY, Bosch RJ, Deeks S, Meneses MV, Lederman MM, Hunt P, Henrich TJ, Marconi VC, Gianella S. Effect of Immune-Modulatory Interventions on Asymptomatic Cytomegalovirus Shedding During Suppressive Antiretroviral Therapy. J Infect Dis. 2023 Jun 28;228(1):64-69. doi: 10.1093/infdis/jiad060. PMID: 36881933; PMCID: PMC10490531
George AF, Roan NR. Advances in HIV Research Using Mass Cytometry. Curr HIV/AIDS Rep. 2023 Apr;20(2):76-85. doi: 10.1007/s11904-023-00649-x. Epub 2023 Jan 23. PMID: 36689119; PMCID: PMC9869313.
Rao E, Taylor J, Kaytes A, Concha-Garcia S, Riggs PK, Smith DM, Dubé K, Gianella S. Vulnerability in Biomedical Research: A Historical Reflection and Practical Implications for HIV Cure-Related Research. AIDS Res Hum Retroviruses. 2024 Jan;40(1):22-27. doi: 10.1089/AID.2022.0136. Epub 2023 Jun 21. PMID: 37227023; PMCID: PMC10790546.
Manca A, De Nicolò A, De Vivo ED, Ferrara M, Oh S, Khalili S, Higgins N, Deiss RG, Bonora S, Cusato J, Palermiti A, Mula J, Gianella S, D’Avolio A. A Novel UHPLC-MS/MS Method for the Quantification of Seven Opioids in Different Human Tissues. Pharmaceuticals (Basel). 2023 Jun 19;16(6):903. doi: ...
Tang Y, Chaillon A, Gianella S, Wong LM, Li D, Simermeyer TL, Porrachia M, Ignacio C, Woodworth B, Zhong D, Du J, de la Parra Polina E, Kirchherr J, Allard B, Clohosey ML, Moeser M, Sondgeroth AL, Whitehill GD, Singh V, Dashti A, Smith DM, Eron JJ, Bar KJ, Chahroudi ...
Ahuja SK, Manoharan MS, Lee GC, McKinnon LR, Meunier JA, Steri M, Harper N, Fiorillo E, Smith AM, Restrepo MI, Branum AP, Bottomley MJ, Orrù V, Jimenez F, Carrillo A, Pandranki L, Winter CA, Winter LA, Gaitan AA, Moreira AG, Walter EA, Silvestri G, King CL, Zheng YT, Zheng HY, ...
Bajaj T, Wehri E, Suryawanshi RK, King E, Pardeshi KS, Behrouzi K, Khodabakhshi Z, Schulze-Gahmen U, Kumar GR, Mofrad MRK, Nomura DK, Ott M, Schaletzky J, Murthy N. Mercapto-pyrimidines are reversible covalent inhibitors of the papain-like protease (PLpro) and inhibit SARS-CoV-2 (SCoV-2) replication. RSC Adv. 2023 Jun 12;13(26):17667-17677. doi: 10.1039/d3ra01915b. ...
Karine Dubé DrPH, Tia Morton RN, Lawrence Fox MD, Lynda Dee JD, David Palm MS, Thomas J Villa, William Freshwater, Jeff Taylor, Gail Graham, William B Carter, John A Sauceda PhD, Michael J Peluso MD, Annette Rid MD
Dubé K, Shelton B, Patel H, Ndukwe SO, Concha-Garcia S, Dullano C, Solso S, Hendrickx S, Kaytes A, Taylor J, Villa TJ, Little SJ, Riggs PK, Lessard D, Arora AK, Costiniuk CT, Eskaf S, Smith DM, Gianella S. Perceived risks and benefits of enrolling people with HIV at the end ...
Dubé K, Villa TJ, Taylor J, Kaytes A, Moore DJ, Little SJ, Chaillon A, Smith DM, Gianella S. A Community-Driven Framework to Prioritize the Use of Donated Human Biological Materials in the Context of HIV Cure-Related Research at the End of Life. Pathog Immun. 2023 May 24;8(1):1-16. doi: 10.20411/pai.v8i1.583. PMID: ...
Ken, M.L., Roy, R., Geng, A. et al. RNA conformational propensities determine cellular activity. Nature 617, 835–841 (2023). https://doi.org/10.1038/s41586-023-06080-x
Oliveira MF, Pankow A, Vollbrecht T, Kumar NM, Cabalero G, Ignacio C, Zhao M, Vitomirov A, Gouaux B, Nakawawa M, Murrell B, Ellis RJ, Gianella S. Evaluation of Archival HIV DNA in Brain and Lymphoid Tissues. J Virol. 2023 Jun 29;97(6):e0054323. doi: 10.1128/jvi.00543-23. Epub 2023 May 15. PMID: 37184401; PMCID: ...
Wanjalla CN, Simmons J, Oakes J, Zhang X, Nochowicz C, Priest S, Bailin SS, Warren CM, Mashayekhi M, Beasley HK, Wang J, Meenderink L, Sheng Q, Stolze J, Gangula R, Chopra A, Gabriel CL, Temu T, Pakala S, Wilfong EM, Gianella S, Phillips EJ, Harrison DG, Hinton A, Kalams SA, ...
Taylor BC, Sheikh Andalibi M, Wandro S, Weldon KC, Sepich-Poore GD, Carpenter CS, Fraraccio S, Franklin D, Iudicello JE, Letendre S, Gianella S, Grant I, Ellis RJ, Heaton RK, Knight R, Swafford AD. Signatures of HIV and Major Depressive Disorder in the Plasma Microbiome. Microorganisms. 2023 Apr 14;11(4):1022. doi: 10.3390/microorganisms11041022. ...
Ling, Lijun; Leda, Ana R; Begum, Nurjahan; Spagnuolo, Rae Ann; Wahl, Angela; Garcia, J Victor; Valente, Susana T. Loss of In Vivo Replication Fitness of HIV-1 Variants Resistant to the Tat Inhibitor, dCA. Viruses2023 Apr 12; 15 (4)
Hamann, M.V., Adiba, M. & Lange, U.C. Confounding factors in profiling of locus-specific human endogenous retrovirus (HERV) transcript signatures in primary T cells using multi-study-derived datasets. BMC Med Genomics 16, 68 (2023). https://doi.org/10.1186/s12920-023-01486-y
Joseph SB, Gianella S, Burdo TH, Cinque P, Gisslen M, Letendre S, Nath A, Morgello S, Ndhlovu LC, Spudich S. Biotypes of Central Nervous System Complications in People With Human Immunodeficiency Virus: Virology, Immunology, and Neuropathology. J Infect Dis. 2023 Mar 17;227(Suppl 1):S3-S15. doi: 10.1093/infdis/jiac370. PMID: 36930640; PMCID: PMC10022721.
Boehm D, Lam V, Schnolzer M, Ott M. The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell Rep. 2023 Mar 9;42(3):112234. doi: 10.1016/j.celrep.2023.112234. Epub ahead of print. PMID: 36897778.
Mukim, Amey; Smith, Davey M; Deshmukh, Savitha; Qazi, Andrew A; Beliakova-Bethell, Nadejda A Camptothetin Analog, Topotecan, Promotes HIV Latency via Interference with HIV Transcription and RNA Splicing. Journal of virology2023 Feb 28; 97 (2) e0163022
Wanjalla CN, Gabriel CL, Fuseini H, Bailin SS, Mashayekhi M, Simmons J, Warren CM, Glass DR, Oakes J, Gangula R, Wilfong E, Priest S, Temu T, Newell EW, Pakala S, Kalams SA, Gianella S, Smith D, Harrison DG, Mallal SA, Koethe JR. CD4+ T cells expressing CX3CR1, GPR56, with variable ...
Kaunda L, Ngoma MS, Menon JA, Heaton RK, Gianella S, Bharti AR, Letendre S, de Oliveira MF, Hestad KA. Effect of coinfections on neurocognitive functioning among people with clade C HIV infection in Zambia. J Neurovirol. 2023 Feb;29(1):45-52. doi: 10.1007/s13365-022-01110-2. Epub 2023 Feb 2. PMID: 36729300; PMCID: PMC10748732.
Packard, Thomas A; Schwarzer, Roland; Herzig, Eytan; Rao, Deepashri; Luo, Xiaoyu; Egedal, Johanne H; Hsiao, Feng; Widera, Marek; Hultquist, Judd F; Grimmett, Zachary W; Messer, Ronald J; Krogan, Nevan J; Deeks, Steven G; Roan, Nadia R; Dittmer, Ulf; Hasenkrug, Kim J; Greene, Warner C. CCL2: a Chemokine Potentially Promoting Early ...
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Srinivasachar Badarinarayan, Smitha; Sauter, Daniel Not all viruses cause disease: HERV-K(HML-2) in healthy human tissues. PLoS biology2022 Oct; 20 (10) e3001884
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Gladwin, D., Horst, R., James, K., & Sameshima, P. (2022).
Yuan Z, Giron LB, Hart C, Gyampoh A, Koshy J, Hong KY, Niki T, Premeaux TA, Ndhlovu LC, Deleage C, Montaner LJ, Abdel-Mohsen M.
Shelli F. Farhadian,1,2 Ofir Lindenbaum,3,4 Jun Zhao,4,5 Michael J. Corley,6,7 Yunju Im,8 Hannah Walsh,1 Alyssa Vecchio,9 Rolando Garcia-Milian,10 Jennifer Chiarella,2 Michelle Chintanaphol,2 Rachela Calvi,2 Guilin Wang,11 Lishomwa C. Ndhlovu,6,7 Jennifer Yoon,1 Diane Trotta,5 Shuangge Ma,8 Yuval Kluger,3,4,12 and Serena Spudich2
Ma T, McGregor M, Giron L, Xie G, George AF, Abdel-Mohsen M, Roan NR.
Kanazawa J, Rawlings SA, Hendrickx S, Gianella S, Concha-Garcia S, Taylor J, Kaytes A, Patel H, Ndukwe S, Little SJ, Smith D, Dubé K. Lessons learned from the Last Gift study: ethical and practical challenges faced while conducting HIV cure-related research at the end of life. J Med Ethics. 2023 ...
Luo X, Frouard J, Zhang G, Neidleman J, Xie G, Sheedy E, Roan NR, Greene WC.
Curty G, Iniguez LP, Soares MA, Nixon DF, de Mulder Rougvie M.
George AF, McGregor M, Gingrich D, Neidleman J, Marquez RS, Young KC, Thanigaivelan KL, Greene WC, Tien PC, Deitchman AN, Spitzer TL, Roan NR.
Li, Jie; Tuma, Jan; Han, Hesong; Kim, Hansol; Wilson, Ross C; Lee, Hye Young; Murthy, Niren The Coiled-Coil Forming Peptide (KVSALKE)5 Is a Cell Penetrating Peptide that Enhances the Intracellular Delivery of Proteins. Advanced healthcare materials2022 May; 11 (9) e2102118
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25. Hardwick, Simon A; Hu, Wen; Joglekar, Anoushka; Fan, Li; Collier, Paul G; Foord, Careen; Balacco, Jennifer; Lanjewar, Samantha; Sampson, Maureen McGuirk; Koopmans, Frank; Prjibelski, Andrey D; Mikheenko, Alla; Belchikov, Natan; Jarroux, Julien; Lucas, Anne Bergstrom; Palkovits, Miklós; Luo, Wenjie; Milner, Teresa A; Ndhlovu, Lishomwa C; Smit, August B; Trojanowski, ...
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