Hello HOPEians,
In Monday’s meeting on June 6th, we heard data updates from the Chemnitz and Lange Labs. They explained the construction and functional characterization of an enzymatically dead Brec1 and the generation of different expression plasmids for brec1 and dBrec1. They also went over CD4-specific AAV2 vectors using nanobody capsid engineering. Priti gave a quick update on using CRISPR-BE (BE4) targeted to TAR to inhibit HIV; initial LTR reporter assays show reduced luciferase expression, next they will test using Ya-chi Ho’s clonal cell lines. Priti shared the paper “Efficient viral delivery of Cas9 into human safe harbor” and Nadia shared “Why drug delivery is the key to new medicines”.
There is a working group meeting on CRISPRoff efforts and relevant data on June 13th at 10am PT. Joining information is on the HOPE calendar.
Here are the updated actions from our last meeting. If you have any questions about the meeting or action items please reach out to Melanie and Priti.
Actions:
- (In progress) Priti have a meeting with Ulrike
- Discuss CD7 nanobodies and adding RSV molecules to AAV for targeted delivery
- Jan – Generate a Brec1 construct using an SFFV promoter for Priti
Long-term Actions:
- (In progress) Priti – Provide mouse tissues to Lish for scRNA-seq
- Lish to set up a pipeline for sequencing and analysis
- Potentially going to Yale to do initial steps on site
- Priti- Continue to work on Brec1 delivery using a lenti or VLP based methods
Announcements:
- The July 4th meeting is canceled for the holiday
- The next meeting is scheduled for Aug 1st and we will host a HOPE – CRISPR for Cure Joint Meeting with Guest Speakers – Dr. Kamel Khalili and Dr. Tricia Burdo