Here is a quick update on the RF1 meeting from July 18th, 2022.
During the RF1 meeting, Cedric Feschotte (Cornell University) shared updates that Sabrina Leddy has started working in his lab on HERV expression in HIV elite controllers and Julius Judd who was previously working on zinc fingers is leaving his lab soon. Susana Valente (University of Florida) explained her research with DUX4, FUBP3, dCA-resistant viruses, and how p32 interacts with Tat’s basic domain.
Zichong Li (Greene Lab at Gladstone) discussed different epigenetic modifiers and the effects of new SPAs and the nucleosomal modification mechanisms for silencing HIV. Martin Kinisu (Greene Lab) also shared his work on CRISPRoffV2 combining DNA and H3K9 methylation, the optimization of KRAB domain for HIV silencing, and exploring inducible expression systems using the piggyback technology. In his early findings, Martin found that ETRs transiently silence HIV reactivation in 30-70% of cells. Melanie Ott and Daniela Boehm (Gladstone) resubmitted their SYMD5 paper and performed the SMYD5 rescue experiment in 5A8 J-Lat cells. Their findings show that SYMD5 is highly expressed in CD4+ T cells and is a positive regulator of HIV transcription. SYMD5 also methylates histones and Tat in vitro.
Actions for next meeting:
Susana Valente – Schedule a meeting with Cedric to go over DUX4 data
Chuan Li – Give MNase-Seq protocol to Zichong Li
Roan Lab – Look for DUX4 expression in single-cell RNAseq tonsil datasets (+/- HIV)
(In progress) Luisa Mori, Ana Leda, and Susana Valente considering writing a review/summary of all the HIV latency models
August 15th HOPE – PAVE Guest Speaker: Dr. Persaud
The next standing meeting is on Sept 19 – Bharath Sreekumar will give a refresher on their data on transducing T cells. Cedric Feschotte and Sabrina Leddy will give updates on HERV expression in HIV-1 elite controllers.
Call for nominations for the HOPE 2022 Awards (Instructions attached)
Please Acknowledge HOPE & the NIH in any publications or presentations and notify the Program Manager. Here are some examples of what you can include.
“Research reported in this publication was supported by the NIAID of the National Institutes of Health under award number UM1AI164559, with co-funding support from NIDA, NIMH, NHLBI, the NIDDK, and the NINDS. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”
“This research was supported by NIAID award number UM1AI164559, co-funded by NHLBI, NIDA, NIMH, NINDS, and NIDDK.”